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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_Lu33
Organism:Mus musculus
UniGene Library ID:3746
Tissue Description:Lung, 2 pooled mouse tumors.
Library Keywords:metastasis, lung, bulk, transgene, normalized, CGAP, EST, Soares normalized, unknown developmental stage, MGC, phagemid, lung tumor, CZECH II

Clones and Sequences

#Clones Generated to Date:21504
#Sequences Generated to Date:14653

Library Preparation Details

Description:1st strand cDNA was prepared from mRNA obtained from pooled lung tumors with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCTGTTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:NotI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Gilbert Smith, Ph.D.
Library Producer:Soares Laboratory