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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Mam10
Organism:Mus musculus
Strain:
UniGene Library ID:4057
Tissue Description:Mammary tumor. 6 pooled mouse mammary tissues.
Library Keywords:mammary gland, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, female, phagemid, oligo-dT primed, breast (mammary gland) cancer

Clones and Sequences

#Clones Generated to Date:1920
#Sequences Generated to Date:1391

Library Preparation Details

Description:1st strand cDNA was prepared from mRNA obtained from pooled mammary gland tumors with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACTAGTTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:NotI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Lothar Hennighausen Ph.D., Jeffrey Green M.D., Gilbert Smith, Ph.D., William Muller, M.D.
Library Producer:Soares Laboratory