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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Barstead mouse pooled organs MPLRB4
Organism:Mus musculus
Strain:FVB/N
UniGene Library ID:609
Tissue Description:
Library Keywords:normal, bulk, EST, unknown developmental stage, mixed tissue, mixed pool by gender, phagemid, oligo-dT primed, FVB/N

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:6988

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [GTTGGATTCGGTACC], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.
R. Site1:EcoRI
R. Site2:NotI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: