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Library Info Page


Library ID

Library Name:RIKEN full-length enriched, 18 days pregnant, placenta and extra embryonic tissue
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:2520
Tissue Description:
Library Keywords:normal, placenta, bulk, pregnant, adult, normalized, subtracted, full-length enriched, EST, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3967

Library Preparation Details

Description:cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 50.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I
R. Site1:SalI
R. Site2:BamHI
Lab Host:DH10B
Vector:
Vector Type:
Tissue Supplier:
Library Producer: