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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares mouse placenta 4NbMP13.5 14.5
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:470
Tissue Description:
Library Keywords:normal, placenta, bulk, adult, normalized, EST, Soares normalized, female, phagemid, oligo-dT primed, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:5883

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: