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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Beddington mouse embryonic region
Organism:Mus musculus
Strain:C57BL/6 x DBA
UniGene Library ID:547
Tissue Description:
Library Keywords:normal, micro-bulk, EST, gastrula, embryonic tissue, mixed pool by gender, size fractionated, directionally cloned, phagemid, oligo-dT primed, crossbred

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:8623

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Gastrulating embryos were collected at 7.5dpc from C57BL6 x DBA matings, excluding embryos that had developed head folds and all extraembryonic tissues. Average insert size: 1.3 kb (range: 0.5 - 3.0 kb). Referenced in Development 121, 2479-2489 (1995)
R. Site1:SalI
R. Site2:NotI
Lab Host:DH12S
Vector:pCMV-SPORT
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: