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Library Info Page


Library ID

Library Name:NIH_BMAP_M_S3.3
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:2490
Tissue Description:Brain, pooled tissue from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus).
Library Keywords:normal, brain stem, cerebellum, cerebral cortex, BMAP, amygdala, bulk, basal ganglia, olfactory bulb, hypothalamus, pineal gland, subtracted, corpus striatum, juvenile, EST, Soares subtracted, phagemid, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2369

Other Libraries from Same Tissue Sample

NIH_BMAP_M_S1
NIH_BMAP_M_S2
NIH_BMAP_M_S3.2
NIH_BMAP_M_S3.1
NIH_BMAP_M_S4
NIH_BMAP_M_S5

Library Preparation Details

Description:The NIH_BMAP_M_S3.3 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S3.3, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S3.3) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S2 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the NIH_BMAP_M_S2 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S3.3 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996)
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (Life Technologies)
Vector:pT7T3D-PacI
Vector Type:Phagemid
Tissue Supplier:Ms. Annie Novakovich, Zivic-Miller Laboratories.
Library Producer:Soares Laboratory