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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:mouse liver, vehicle control
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:2484
Tissue Description:
Library Keywords:liver, normal, non-normalized, bulk, EST, unknown developmental stage, male, plasmid vector, oligo-dT primed, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2250

Library Preparation Details

Description:First strand cDNA was primed with a NotI-polyT primer [5'-AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT-3']. Double-stranded cDNA was ligated with EcoRI adapters (Pharmacia), digested with NotI, and ligated into the EcoRI/NotI sites of the pGEM11Zf vector. The library was NOT normalized.
R. Site1:NotI
R. Site2:EcoRI
Lab Host:JM109
Vector:pGEM11Zf (Promega)
Vector Type:plasmid
Tissue Supplier:
Library Producer: