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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Soares mouse p3NMF19.5
Organism:Mus musculus
Strain:
UniGene Library ID:431
Tissue Description:
Library Keywords:fetus, normal, micro-bulk, normalized, EST, Soares normalized, embryonic tissue, size fractionated, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:27051

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University).
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:phagemid
Tissue Supplier:
Library Producer: