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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares mouse embryo NbME13.5 14.5
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:437
Tissue Description:
Library Keywords:fetus, normal, micro-bulk, normalized, EST, Soares normalized, embryonic tissue, size fractionated, oligo-dT primed, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:42540

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTTT 3'], on equal amounts of mRNA from 2 13.5dpc and 2 14.5dpc embryos [total RNA provided by Minoru Ko, Wayne State Univ., from 2 ]; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: