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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Abe mouse ES cell
Organism:Mus musculus
Strain:
UniGene Library ID:21037
Tissue Description:
Library Keywords:cell line, embryonic stem cell, embryonic tissue, unknown embryonic stage, oligo-dT primed, EST

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:95566

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-CAGCGGCCGCTTTTTTTTTTTTTTTTTTV-3']; double-stranded cDNA was ligated to Sal I adaptors [5'-TCGACCCACGCGTCCG-3'], digested with Not I and cloned into the Sal I and Not I sites of pSPORT 1 vector.; Average insert size: 0.7 kb (range 0.2-1.3 kb); Sequence primer: T7 [5'-TAATACGACTCACTATAGGG-3']; Primary library, non-amplified.
R. Site1:Sal I
R. Site2:Not I
Lab Host:
Vector:pSPORT 1 (GibcoBRL)
Vector Type:
Tissue Supplier:
Library Producer: