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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:MacDonald Sertoli cells
Organism:Mus musculus
UniGene Library ID:20540
Tissue Description:
Library Keywords:testis, juvenile, EST, Sertoli cell, size fractionated, plasmid vector, directionally cloned, oligo-dT primed, CD-1

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:276

Library Preparation Details

Description:Primary, unamplified cDNA libraries from 18-20 day mouse Sertoli cells (10 pfu/ul) were constructed in lambda Uni-Zap XR (Stratagene, CA) as directionally cloned from oligo(dT)-primed cDNA from Swiss (CD-1) mouse testis poly(A)+ RNA (See McCarrey, et al. J. Androl. 1999 20:635-639). Plasmid rescues were performed by mass excision on these libraries according to the manufacturer's protocol (Stratagene, CA). cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of > 400bp material gives average insert size ranging from 1-2 kb. Original lambda-based library is available through ATCC, catalog #63427.
R. Site1:XhoI
R. Site2:EcoRI
Lab Host:Escherichia coli (XL2 Blue MRF')
Vector:Stratagene lambda Uni-Zap XR
Vector Type:Plasmid
Tissue Supplier:
Library Producer: