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Library Info Page


Library ID

Library Name:FVB mouse degenerating retinas, unamplified (nat)
Organism:Mus musculus
Strain:FVB
UniGene Library ID:18934
Tissue Description:
Library Keywords:retina, juvenile, EST, plasmid vector, directionally cloned, FVB

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1470

Library Preparation Details

Description:RNA was extracted from 5 weeks old FVB retinas. A directionally cloned cDNA library in the pBluescript IIXR vector (Stratagene) was constructed following the protocols of the Library Construction Kit System full details of which are contained in the manufacturer's Instruction manual. cDNA was cloned in EcoRI /Xho I sites such as T7 is the antisense and T3 is the sense primer. Sequencing and informatics was performed through the NEIBank project (http://neibank.nei.nih.gov).
R. Site1:
R. Site2:
Lab Host:Epicurian Coli XL 10-Gold
Vector:pBluescript II XR
Vector Type:Plasmid
Tissue Supplier:
Library Producer: