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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Eppig Hampl Solter fully-grown mouse oocyte
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:18552
Tissue Description:
Library Keywords:EST, oocyte, female, size fractionated, C57BL/6J, primary oocyte

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:18790

Library Preparation Details

Description:Cloned unidirectionally from mRNA prepared from fully-grown germinal vesicle stage oocytes isolated 46 hours after PMSG injection. NotI(dT) primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. SalI linker: 5'-TCGACCCACGCGTCCG-3'. cDNAs were size-selected for 500 bp and cloned into the SalI/NotI sites of pSPORT vector (Life Technologies). Average insert size: 1.5 kb. I.M.A.G.E. library ID 1182.
R. Site1:SalI (5')
R. Site2:NotI (3')
Lab Host:DH10B
Vector:pSPORT1
Vector Type:phagemid
Tissue Supplier:
Library Producer: