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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Mouse total cornea. Unnormalized
Organism:Mus musculus
Strain:C57Black
UniGene Library ID:18292
Tissue Description:
Library Keywords:normal, non-normalized, cornea, juvenile, EST, plasmid vector, oligo-dT primed, C57Black

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1114

Library Preparation Details

Description:Total corneas were dissected from 40 mouse eyes under a dissection microscope. Total RNA was isolated from the indicated tissues (RNazol; TelTest, Friendswood, TX). Total RNA (300 ug) was used for construction of the library. Poly(A)+RNA was prepared using Dynabeads Oligo (dT)25 (Invitrogen, Carlsbad, CA). The cDNA was synthesized and directionally cloned in the Uni-ZAP XR vector (Stratagene, La Jolla, CA). First strand synthesis was carried out using an oligo(dT) linker-primer containing an Xho I site [5'-(GA)10-ACTAGTCTCGAG(T)18-3']. cDNA was cloned in EcoR I-Xho I sites. In vivo excision releases the pBluescript SK(+/-) phagemid. Templates were sequenced using the Beckman CEQ2000 sequencing kit on a CEQ2000 Genomic Analysis System (Beckman-Coulter, Fullerton, CA). Analyzed data available through http://neibank.nei.nih.gov. NbLib0115: NEI mouse cornea (adult) unnormalized (bn1)
R. Site1:
R. Site2:
Lab Host:
Vector:pBluescript SK(+/-)
Vector Type:Plasmid
Tissue Supplier:
Library Producer: