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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:FH WT129lig05
Organism:Mus musculus
Strain:120
UniGene Library ID:18151
Tissue Description:
Library Keywords:prostate, normal, adult, EST, male, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:436

Library Preparation Details

Description:Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled coagulating glands, ventral prostate, and dorsolateral prostate was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 22 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled mouse prostate from ten adult male mice by Colin Pritchard.
R. Site1:SfiI
R. Site2:SfiI
Lab Host:BM25.8
Vector:pTriplEx2
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: