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Library Info Page


Library ID

Library Name:FH UGS01
Organism:Mus musculus
Strain:C57BL6
UniGene Library ID:18148
Tissue Description:
Library Keywords:fetus, EST, urogenital sinus, mixed pool by gender, plasmid vector, C57BL/6

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3271

Library Preparation Details

Description:Library was constructed using Clontechs SMART cDNA library construction kit. 1ug of total RNA from pooled urogenital sinuses was used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 18 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled urogenital sinuses from E16 mouse embryos (male and female) by Colin Pritchard.
R. Site1:SfiI
R. Site2:SfiI
Lab Host:BM25.8
Vector:pTriplEx2
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: