Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:FH NEONATAL
Organism:Mus musculus
UniGene Library ID:18147
Tissue Description:
Library Keywords:prostate, neonate, EST, male, plasmid vector, C57BL/6

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:5963

Library Preparation Details

Description:Library was constructed using Clontechs SMART cDNA library construction kit. 0.1ug of total RNA from pooled 2-day dorsolateral prostate, 0.1ug of total RNA from pooled 2-day ventral prostate, and 0.1ug of total RNA from pooled 2-day coagulating gland (anterior prostate) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 23 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five two day old male C57BL6 mice by Colin Pritchard.
R. Site1:SfiI
R. Site2:SfiI
Lab Host:BM25.8
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: