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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:FH MONTH14
Organism:Mus musculus
Strain:C57BL6
UniGene Library ID:18146
Tissue Description:
Library Keywords:prostate, normal, adult, EST, male, plasmid vector, C57BL/6

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3385

Library Preparation Details

Description:Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled 14-month dorsolateral prostate, 0.5ug of total RNA from pooled 14-month ventral prostate, and 0.5ug of total RNA from pooled 14 month coagulating gland (anterior prostate) was DNAseI treated, combined and used in 1st strand cDNA synthesis. The RNA from the coagulating gland was partially degraded. 2nd strand cDNA was generated by 23 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from 14 month old male C57BL6 mice by Colin Pritchard.
R. Site1:SfiI
R. Site2:SfiI
Lab Host:BM25.8
Vector:pTriplEx2
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: