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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:FH DAY35S
Organism:Mus musculus
Strain:C57BL6
UniGene Library ID:18144
Tissue Description:
Library Keywords:prostate, normal, juvenile, EST, male, plasmid vector, C57BL/6

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2331

Library Preparation Details

Description:Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled 35-day dorsolateral prostate, 0.5ug of total RNA from pooled 35-day ventral prostate, and 0.5ug of total RNA from pooled 35-day coagulating gland (anterior prostate ) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR. One prominent band from the double stranded cDNA was removed from the gel and the remaining cDNA was gel isolated. This subtracted cDNA was digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five 35 day old male C57BL6 mice by Colin Pritchard.
R. Site1:SfiI
R. Site2:SfiI
Lab Host:BM25.8
Vector:pTriplEx2
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: