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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:FH DAY20
Organism:Mus musculus
Strain:C57BL6
UniGene Library ID:18142
Tissue Description:
Library Keywords:prostate, normal, juvenile, EST, male, plasmid vector, C57BL/6

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3969

Library Preparation Details

Description:Library was constructed using Clontechs SMART cDNA library construction kit. RNA was DNase treated prior to 1st strand cDNA synthesis. 0.33ug of total RNA from pooled 20- day dorsolateral prostate, 0.33ug of total RNA from pooled 20-day ventral prostate, and 0.33ug of total RNA from pooled 20-day coagulating gland (anterior prostate) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR, digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate lobes from five 20-day old mice by Colin Pritchard.
R. Site1:SfiI
R. Site2:SfiI
Lab Host:BM25.8
Vector:pTriplEx2
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: