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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:5' RACE cDNAs from cytosolic mouse RNA
Organism:Mus musculus
Strain:
UniGene Library ID:15579
Tissue Description:
Library Keywords:EST, embryonic tissue, mixed embryonic stages, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:180

Library Preparation Details

Description:Total cytosolic mouse RNA from : 7, 11, 15, & 17-day total embryo, whole brain, eye, kidney, liver, lung, prostate, submaxilary gland, smooth muscle, spleen, testes and uterus were divided into 4 pools with a maximum of 4 tissues/stages per pool. After 5' RACE cDNA generation, the cDNA for all the samples were combined and used in RACE-PCR amplification. Amplification of 5' RACE cDNA was carried out using nested gene-specific primers and adapter specific primers. After amplification, the products were cloned and sequenced from both ends using universal primers. 5'->3' cDNA sequence was artificially generated by joining the sequences from the ends for each clone.
R. Site1:
R. Site2:
Lab Host:
Vector:pCR-TOPO2.1
Vector Type:plasmid
Tissue Supplier:
Library Producer: