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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_230
Organism:Mus musculus
Strain:
UniGene Library ID:15414
Tissue Description:Thyroid, mouse (Black Swiss/Taconic), 4-5 weeks, normal wild-type, pooled from 5 mice
Library Keywords:thyroid, normal, non-normalized, bulk, juvenile, EST, MGC, size fractionated, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:4992
#Sequences Generated to Date:4546

Library Preparation Details

Description:RNA obtained from 5 normal wild-type mice thyroid. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4 kb resulted in an average insert size of 1.2 kb. Normalized version of this library is NIH_MGC_189ibrary constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC Library.
R. Site1:NotI
R. Site2:NotI
Lab Host:DH10B TonA
Vector:pExpress-1
Vector Type:plasmid
Tissue Supplier:Shioko Kimura/Atsushi Yamada, (NCI,CCR)
Library Producer:Express Genomics