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Library Info Page


Library ID

Library Name:MTA adult mouse thymus library
Organism:Mus musculus
Strain:C57BL/6
UniGene Library ID:391
Tissue Description:
Library Keywords:thymus, normal, bulk, adult, EST, size fractionated, plasmid vector, directionally cloned, oligo-dT primed, C57BL/6

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:293

Library Preparation Details

Description:The cDNA library was constructed from poly(A)+ RNA of an adult mouse thymus by oligo-dT primed reverse transcription. cDNA was selected on gel for size above 800 nucleotides after second strand synthesis, then directionally cloned after adaptor ligation and restriction enzyme cleavage into the pcDNA1 vector (Not 1 on polyA side, EcoR 1 on the 5'side).
R. Site1:Not1
R. Site2:EcoR1
Lab Host:MC1061 p3
Vector:pcDNA1
Vector Type:Plasmid
Tissue Supplier:
Library Producer: