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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_BMAP_HE0
Organism:Mus musculus
UniGene Library ID:14477
Tissue Description:Eye
Library Keywords:fetus, eye, normal, non-normalized, BMAP, bulk, EST, MGC, plasmid vector, directionally cloned, oligo-dT primed, C57BL/6

Clones and Sequences

#Clones Generated to Date:3072
#Sequences Generated to Date:2599

Library Preparation Details

Description: The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site .Double strand cDNA was size selected according to mRNA size fraction ,ligated with EcoR I adaptor , digested with NotI and then cloned directionally into pYX-Asc vector . The library tag sequence located between the Not I site and the polyA tail is TTATTGAAGT. This library was created for the University Iowa Brain Anatomy Project (BMAP): "Gene Discovery in the Developing Mouse Nervous System", supported by National Institute of Mental Health (NIMH).
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (T1 phage resistant)
Vector:pYX- Asc
Vector Type:Plasmid
Tissue Supplier:Dr. James Lin, University of Iowa
Library Producer:Soares Laboratory