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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Organ of Corti
Organism:Mus musculus
Strain:
UniGene Library ID:14415
Tissue Description:
Library Keywords:normal, bulk, juvenile, EST, plasmid vector, organ of Corti

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1199

Library Preparation Details

Description:The organ of Corti (OC) was fine dissected from a total of 386 OC as follows: 102 samples from post-natal (P) day 5; 72 from P6; 60 from P7; 46 from P8; 18 from P9; 20 from P10; 14 from P12 and 24 from P13. Total RNA was extracted using the micro Fasttrack kit (catalog # K1593-02; Invitrogen, Carlsbad, CA), according to manufacturer's instructions. Reverse transcription and library construction were carried out with the Uni-Zap XR vector kit (catalog # 237211, Stratagene) and Uni-Zap XR Gigapack III Gold Cloning kit (catalog # 237612), both from Stratagene (La Jolla, CA, USA), according to manufacturer's instructions. The frequency distribution of the library is as follows: 72% of genes have 1 copy; 14.3% 2; 12% 3-10; 1.4% 11-50 and 0.1% 51-150. As to gene function, 45% of genes are present in GenBank and have know function; 23% have hits in GenBank,but do not have assigned function; 12% are uncharacterized ESTs and 20% are unidentified. Library created in the laboratory of M. Brownstein (NIMH, NIH). A complete library decription can be found at .
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:DH10B (phage-resistant)
Vector:pBluescriptSK-
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: