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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_222
Organism:Mus musculus
Strain:
UniGene Library ID:14413
Tissue Description:Placenta, mouse, C57 Black/ 6 Ncr, 16th day of pregnancy
Library Keywords:normal, non-normalized, placenta, bulk, pregnant, adult, EST, MGC, female, size fractionated, plasmid vector

Clones and Sequences

#Clones Generated to Date:7296
#Sequences Generated to Date:6150

Library Preparation Details

Description:RNA obtained from three placentas from female C57/BL6 mouse at 16 days pregnancy. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1 kb resulted in an average insert size of 1.5 kb. Library is not amplified. (Normalized version of this library is NIH_MGC_203.) Library constructed by Express Genomics (Frederick, MD).
R. Site1:EcoRV
R. Site2:NotI
Lab Host:DH10B (phage-resistant)
Vector:pExpress-1
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:Naryan Bhat
Library Producer:Express Genomics