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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Normalized colon epithelial progenitors cDNA library
Organism:Mus musculus
Strain:
UniGene Library ID:14193
Tissue Description:
Library Keywords:normal, colon, microdissected, adult, normalized, EST, phagemid, C57BL/6, epithelial progenitor

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:343

Library Preparation Details

Description:1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Library materials provided by T.S. Stappenbeck and J.I. Gordon. Library constructed by R. Tidwell Y.Korshunova, M. Lovett. Library was re-arrayed by R. Tidwell.
R. Site1:
R. Site2:
Lab Host:XL1 Blue MRF'
Vector:pAMP1
Vector Type:phagemid
Tissue Supplier:
Library Producer: