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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Barstead stromal cell line MPL-RB5
Organism:Mus musculus
Strain:
UniGene Library ID:13912
Tissue Description:
Library Keywords:muscle, normal, cell line, EST, unknown developmental stage, myoblast, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:295

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo (dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTT TTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [AATTCGGATCCTTG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation). The C2C12 cell line (available from ATCC, catalog # CRL-1772) differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:
Vector Type:
Tissue Supplier:
Library Producer: