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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Colon epithelia progenitors cDNA
Organism:Mus musculus
Strain:
UniGene Library ID:13846
Tissue Description:
Library Keywords:normal, colon, uncharacterized preparation, EST, unknown developmental stage, epithelial progenitor

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:11670

Library Preparation Details

Description:1st strand of cDNA was synthesized with reverse transcriptase and oligo(dT) beads, then cDNA was amplified by PCR using modified SMART primers. The final cDNA was cloned in pAMP1 vector in annealing reaction with Uracil DNA Glycosylase (UDG). Library constructed by Y.Korshunova and M. Lovett. Library materials provided by J. Gordon lab.
R. Site1:
R. Site2:
Lab Host:DH5alpha
Vector:pAMP1
Vector Type:bacterial
Tissue Supplier:
Library Producer: