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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_BMAP_GV0
Organism:Mus musculus
Strain:C57BL/6
UniGene Library ID:13962
Tissue Description:Brain
Library Keywords:normal, BMAP, bulk, juvenile, neonate, EST, MGC, mixed developmental stages, plasmid vector, directionally cloned, oligo-dT primed, C57BL/6, whole brain

Clones and Sequences

#Clones Generated to Date:8064
#Sequences Generated to Date:6875

Library Preparation Details

Description:The library was constructed according Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured RNA was size fractionated on a 1% agarose gel.First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according to mRNA size fraction,ligated with EcoR I adaptor, digested with NotI and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail is CGAACTGAAT. This library was created for the University Iowa Brain Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Institute of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (T1 phage resistant)
Vector:pYX- Asc
Vector Type:Plasmid
Tissue Supplier:Dr. Jim Lin, University of Iowa
Library Producer:Soares Laboratory