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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_190
Organism:Mus musculus
Strain:
UniGene Library ID:13939
Tissue Description:Molar, pooled, newborn mouse, strain ICR. First molars, tooth, including ameloblasts and odontoblast.
Library Keywords:normal, non-normalized, bulk, full-length enriched, EST, unknown developmental stage, MGC, molar, plasmid vector

Clones and Sequences

#Clones Generated to Date:4992
#Sequences Generated to Date:4412

Library Preparation Details

Description:Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.71 kb (range 0.5-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA) Corp.
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:Dr. Yoshihiko Yamada, NIDCR, acknowledge-Dr.Takashi Nakamura, NIDCR
Library Producer:CLONTECH Laboratories, Inc.