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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NICHD_MM_Hyp1
Organism:Mus musculus
Strain:
UniGene Library ID:13938
Tissue Description:Brain, normal mediobasal hypothalamus. Mouse C57 Black 6; 8 weeks of age; mixed gender - equal numbers of male and randomly cycling female mice.
Library Keywords:normal, non-normalized, bulk, hypothalamus, full-length enriched, CGAP, EST, Soares subtracted, unknown developmental stage, MGC, plasmid vector

Clones and Sequences

#Clones Generated to Date:2304
#Sequences Generated to Date:1953

Library Preparation Details

Description:Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.08 kb (range .73-1.37 kb). 13/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp.
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:Dr. Pamela Mellon and Dr. Pat Chappell
Library Producer:CLONTECH