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Library Info Page


Library ID

Library Name:NIH_BMAP_M_S2
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:1952
Tissue Description:Brain, pooled tissue from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus).
Library Keywords:normal, brain stem, cerebellum, cerebral cortex, BMAP, amygdala, bulk, basal ganglia, olfactory bulb, hypothalamus, pineal gland, subtracted, corpus striatum, juvenile, EST, Soares subtracted, mixed tissue, phagemid, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:9378

Other Libraries from Same Tissue Sample

NIH_BMAP_M_S1
NIH_BMAP_M_S3.2
NIH_BMAP_M_S3.3
NIH_BMAP_M_S3.1
NIH_BMAP_M_S4
NIH_BMAP_M_S5

Library Preparation Details

Description:The NIH_BMAP_M_S2 library is a subtracted library derived from NIH_BMAP_M_S1, which in turn is a subtracted library derived from a mixture of normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus). The driver used for subtraction consisted of a pool of 5,000 clones from the NIH_BMAP_M_S1 library and a pool of 2,000 clones obtained from non-normalized and normalized mouse brain spinal cord libraries.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (Life Technologies)
Vector:pT7T3D-PacI
Vector Type:Phagemid
Tissue Supplier:Ms. Annie Novakovich, Zivic-Miller Laboratories.
Library Producer:Soares Laboratory