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Library Info Page


Library ID

Library Name:NIH_BMAP_MPG
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:1943
Tissue Description:Brain, mouse pineal gland.
Library Keywords:normal, non-normalized, BMAP, bulk, pineal gland, juvenile, EST, Soares non-normalized, phagemid, oligo-dT primed, C57BL/6J

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:2987

Library Preparation Details

Description:The NIH_BMAP_MPG library is a non-normalized library constructed from mouse pineal gland. The tag is a string of 5 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996. Tissue provided by Ms. Annie Novakovich, Zivic-Miller Laboratories.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (Life Technologies)
Vector:pT7T3D-PacI
Vector Type:Phagemid
Tissue Supplier:Ms. Annie Novakovich, Zivic-Miller Laboratories.
Library Producer:Soares Laboratory