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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_165
Organism:Mus musculus
Strain:
UniGene Library ID:12732
Tissue Description:Testes, mouse, CD-1, age 15 day, primary cultures of Sertoli cells isolated from testes of 15-day-old mice.
Library Keywords:testis, normal, non-normalized, cell line, full-length enriched, EST, Sertoli cell, unknown developmental stage, MGC, Clontech non-normalized, plasmid vector

Clones and Sequences

#Clones Generated to Date:11520
#Sequences Generated to Date:9465

Library Preparation Details

Description:5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.4 kb (range 0.6-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1-phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:Dr. Leslie L. Heckert
Library Producer:CLONTECH Laboratories, Inc.