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Library Info Page


Library ID

Library Name:NIH_MGC_136
Organism:Mus musculus
Strain:
UniGene Library ID:12733
Tissue Description:Jaw and Limb, mouse, day 18.5 to new born, pool of mature fore and hind limb, maxilla and mandible containing endochondral and membraneous bone, formed joints, tendon, ligaments, dermis, epidermis, muscle and teeth with newly forming dentin and enamel
Library Keywords:fetus, normal, bulk, normalized, neonate, EST, LTI normalized, MGC, embryonic tissue, size fractionated, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:8448
#Sequences Generated to Date:6385

Library Preparation Details

Description:Normalized, full-length enriched library from pool of mouse embronic limb, maxilla and mandible, embryonic day 17.5, 18.5 and newborn (mandible (5, 4 and 1 limb and jaw equivalents from respective days). Cloned directionally, oligo-dT primed (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3'. Size selected for the >1kb fragments, average insert size 1.2 kb. Normalization to Cot 7.5 . Tissue contributed by David Rowe; library constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.
R. Site1:EcoRV
R. Site2:NotI
Lab Host:DH10B (phage-resistant)
Vector:pCMV-SPORT6.1
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Dr. David Rowe
Library Producer:Invitrogen Corp