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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_MGC_166
Organism:Mus musculus
UniGene Library ID:12634
Tissue Description:Pituitary, 120 pooled pituitary glands from mouse (CD-1 adult females)
Library Keywords:normal, non-normalized, pituitary gland, bulk, full-length enriched, EST, unknown developmental stage, MGC, Clontech non-normalized, plasmid vector

Clones and Sequences

#Clones Generated to Date:9216
#Sequences Generated to Date:7167

Library Preparation Details

Description:Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.05 kb (range 1.0-4.0 kb). 15/15 colonies contained inserts by PCR.This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp..
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (phage-resistant)
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:T. Rajendra Kumar and Martin M. Matzuk
Library Producer:CLONTECH Laboratories, Inc.