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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:cDNA from mouse aorta
Organism:Mus musculus
Strain:ApoE-/- and LDLR-/- on C57BL/6x 129 background
UniGene Library ID:12138
Tissue Description:
Library Keywords:normal, bulk, adult, subtracted, EST, male, PCR directed strategy, cDNA template, aorta

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:3765

Library Preparation Details

Description:CDNA was prepared from whole aorta divided in atherosclerotic plaque prone regions (aortic arch and abdominal aorta proximal part) and less plaque prone regions (descending thoracic aorta and abdominal aorta distal part). CDNA was fragmented with DpnII, linker ligated and amplified to generated starting material for representational difference analysis (RDA). The two cDNA pools were subjected to iterative RDA subtraction and amplification to enrich for gene fragments differentially expressed at early stages of atherosclerosis.
R. Site1:DpnII
R. Site2:DpnII
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