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Library Info Page


Library ID

Library Name:Hematopoietic Stem Cell Subtracted Library
Organism:Mus musculus
Strain:C57Bl/6J
UniGene Library ID:11946
Tissue Description:
Library Keywords:fetus, liver, normal, flow-sorted, subtracted, hematopoietic cell, EST, plasmid vector, directionally cloned

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:5906

Library Preparation Details

Description:Two directionally cloned cDNA libraries were made from fetal liver hematopoietic stem cells enriched to be Lineageneg/lo, AA4.1pos, cKitpos, Ly6A/E (Sca-1)pos called Scapos and from AA4.1neg fetal liver cells. Subtractive hybridization was performed by hybridization of the target, Scapos, single stranded cDNA library in pSport1 to biotinylated RNA transcribed from the driver, AA4.1neg cDNA library in pSport2 with inserts cloned in the complementary orientation. For detailed protocols and additional information please see our website at http://stemcell.princeton.edu.
R. Site1:Sal I
R. Site2:Not I
Lab Host:DH10B
Vector:Sport 1
Vector Type:Plasmid
Tissue Supplier:
Library Producer: