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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_169
Organism:Mus musculus
Strain:
UniGene Library ID:11976
Tissue Description:Testis, mouse
Library Keywords:testis, normal, non-normalized, bulk, EST, unknown developmental stage, MGC, Clontech non-normalized, male, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:18816
#Sequences Generated to Date:15823

Library Preparation Details

Description:cDNA made by oligo-dT priming and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5 kb size fraction. Library created in thelaboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library.
R. Site1:SfiI (ggccattatggcc)
R. Site2:SfiI (ggccgcctcggcc)
Lab Host:DH10B (T1-phage-resistant)
Vector:pDNR-LIB
Vector Type:plasmid (chloramphenicol resistant, grown at 30C)
Tissue Supplier:Dr. Jonathan Kuo, NIMH
Library Producer:Michael Brownstein / Ted Usdin Laboratory