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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIH_MGC_152
Organism:Mus musculus
Strain:
UniGene Library ID:12109
Tissue Description:Liver, 3 pooled, DMSO treated
Library Keywords:liver, normal, non-normalized, bulk, full-length enriched, EST, DMSO, unknown developmental stage, MGC, directionally cloned, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:10368
#Sequences Generated to Date:7475

Library Preparation Details

Description:cDNA made by oligo-dT with attB2 site and directionally cloned. Priming sequence: 5'-TTTCCTGCAGGCCGGCCACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTT-3'. Full-length enriched library was constructed using the GeneRacer kit by Invitrogen, library amplification 16 cycles. Library constucted by Mark Bittinger in the Bradfield laboratory (McArdle Laboratory for Cancer Research, University of Wisconsin). Note: this is a NIH_MGC Library.
R. Site1:attP2
R. Site2:attP1
Lab Host:DH10B (phage-resistant)
Vector:pDONR201
Vector Type:phagemid (Kanamycin resistant)
Tissue Supplier:Bradfield Laboratory
Library Producer:Chris Bradfield