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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_134
Organism:Mus musculus
Strain:
UniGene Library ID:11140
Tissue Description:Limb, mouse, day 10.5 and 11.5, pool of undifferentiated limb containing undifferentiated limb mesenchyme and early condensing mesenchyme.
Library Keywords:normal, non-normalized, bulk, EST, LTI non-normalized, MGC, directionally cloned, phagemid, oligo-dT primed, organogenesis, embryonic limb

Clones and Sequences

#Clones Generated to Date:14208
#Sequences Generated to Date:12632

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.
R. Site1:EcoRV
R. Site2:NotI
Lab Host:DH10B (phage-resistant)
Vector:pCMV-SPORT6.1
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Dr. David Rowe
Library Producer:Invitrogen Corp