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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:NIH_MGC_137
Organism:Mus musculus
Strain:
UniGene Library ID:11262
Tissue Description:Pancreas, pooled libraries Melton normalized mixed mouse pancreas 1 N1-MMS1, Amplified Melton mou se islets 1 MIS1-A, and Kaestner ngn3 wt.
Library Keywords:pancreas, normal, non-normalized, bulk, EST, unknown developmental stage, MGC, clone collection, phagemid

Clones and Sequences

#Clones Generated to Date:6048
#Sequences Generated to Date:8486

Library Preparation Details

Description:Library consists of a pool of clones rearrayed from the following libraries: Melton normalized mixed mouse pancreas 1 N1-MMS1, Amplified Melton mouse islets 1 MIS1-A, and Kaestner ngn3 wt. Clones rearrayed in the laboratory of K. Kaestner (University of Pennsylvania). Note: this is a NIH_MGC Library.
R. Site1:SalI
R. Site2:NotI
Lab Host:DH10B
Vector:pSPORT1
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Gerard Gradwohl (PNAS 97 P1607-1611, 2000)
Library Producer:Catherine Lee, Endocrine Pancreas Consortium