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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_PlTr1
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:10407
Tissue Description:Placenta, trophoblast, mouse, derived from NCI_CGAP_Pl8, NCI_CGAP_Pl9, NCI_CGAP_Tr1
Library Keywords:normal, bulk, pregnant, adult, subtracted, CGAP, EST, Soares subtracted, female, plasmid vector, directionally cloned, oligo-dT primed, C57BL/6J, trophoblast

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:5721

Library Preparation Details

Description:NCI_CGAP_PlTr1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are GTGTG, ATCAT, GGGTG, GGTTG. For additional information, contact: Bento Soares, bento-soares@uiowa.edu
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (Life Technologies)
Vector:pT7T3-Pac (Pharmacia) with a modified polylinker
Vector Type:Plasmid
Tissue Supplier:Dr. Vera M. da Costa Soares, Dr. Minoru Ko, Dr. Tilo Kunath
Library Producer:Soares Laboratory