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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Kaestner wt amplified
Organism:Mus musculus
Strain:129/Sv x CD1
UniGene Library ID:10350
Tissue Description:
Library Keywords:fetus, pancreas, normal, bulk, full-length enriched, EST, size fractionated, plasmid vector, directionally cloned, oligo-dT primed, crossbred

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:1255

Library Preparation Details

Description:The SMARTtm PCR cDNA synthesis Kit (CLONETECH) was used to obtain full-length cDNA from E14.5 wildtype mRNA. A modified 3' oligo(dT) containing a NotI site was used to prime the first-strand synthesis reaction to allow directional cloning. This full-length cDNA was used for PCR amplification to generate ds cDNA. After size selection (>1kb), SalI adapters were ligated onto both ends of the control cDNA, digested with NotI and cloned into pSPORT1 vector (LTI). The library was electroporated into DH12S cells (LTI) and semi-solid amplified. E14.5 pancreatic primordium was a gift from Dr. Gradwohl.
R. Site1:Not I
R. Site2:Sal I
Lab Host:DH12S
Vector:pSPORT1 (GIBCO)
Vector Type:Plasmid (Amp resistant)
Tissue Supplier:
Library Producer: