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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NIH_BMAP_EQ0
Organism:Mus musculus
UniGene Library ID:10389
Tissue Description:Brain, whole, 18.5 dpc mouse embryo
Library Keywords:fetus, normal, non-normalized, BMAP, bulk, EST, Soares non-normalized, MGC, size fractionated, plasmid vector, directionally cloned, oligo-dT primed, C57BL/6, whole brain

Clones and Sequences

#Clones Generated to Date:1920
#Sequences Generated to Date:1715

Library Preparation Details

Description:The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. Denatured mRNa was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was size selected according to mRNA size fraction, ligated with EcoR I adaptor, digested with Not I, and then cloned directionally into pYX-Asc vector. The library tag sequence located between the Not I site and the polyA tail, is CAGCCACGAC. This library was created for the University of Iowa Mouse Brain Molecular Anatomy Project (BMAP): 'Gene Discovery in the Developing Mouse Nervous System', supported by National Instututes of Mental Health (NIMH), Hemin Chin, Ph.D., program coordinator.
R. Site1:EcoR I
R. Site2:Not I
Lab Host:DH10B (T1 phage resistant)
Vector Type:Plasmid
Tissue Supplier:Dr. James Lin
Library Producer:Soares Laboratory