Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NIA Mouse Newborn Brain cDNA Library
Organism:Mus musculus
Strain:C57BL/6J
UniGene Library ID:9978
Tissue Description:
Library Keywords:normal, bulk, neonate, EST, plasmid vector, oligo-dT primed, C57BL/6J, whole brain

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:4839

Library Preparation Details

Description:Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 48 microgram of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127:1737-1749 (2000) [PMID:10725249]), purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were digested with SalI and NotI enzymes, and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. Coli host was trasnformed with ligation mixture by the standard chemical method. The average insert size is about 1.9 kb. The library was constructed by Yulan Piao (NIA).
R. Site1:SalI
R. Site2:NotI
Lab Host:DH10B
Vector:pSPORT1 (Invitrogen)
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: