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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Melton Normalized Mixed Mouse Pancreas 1 N1-MMS1
Organism:Mus musculus
UniGene Library ID:9658
Tissue Description:
Library Keywords:pancreas, normal, bulk, normalized, EST, Soares normalized, mixed developmental stages, mixed pool by gender, size fractionated, plasmid vector, oligo-dT primed, ICR

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:24239

Library Preparation Details

Description:Five libraries representing EI0.5/12.5 pancreatic bud, E16.5 pancreas, newborn pancreas, adult pancreas, and adult islets of Langerhans were seperately constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA was made by oligo-dT priming and size-selected by column fractionation. Libraries were amplified once on solid support and plasmid DNA from each library was prepared and mixed in equal amounts. The mixed library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares 1996 Genome Research 6:791-806; 0.5 microgram single-stranded mixed library plasmid DNA was mixed with 5 micrograms PCR product representing mixed library inserts and hybridized to an Ecot of 6. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library.
R. Site1:Not I
R. Site2:Sal I
Lab Host:DH10B
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: