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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Kaestner ngn3 - -
Organism:Mus musculus
Strain:129/Sv x CD1
UniGene Library ID:8904
Tissue Description:
Library Keywords:fetus, pancreas, normal, bulk, EST, plasmid vector

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:4015

Library Preparation Details

Description:The library was prepared by Catherine S. Lee and has not been published. The pancreas was obtained from Gerard Gradwohl (PNAS 97 P1607-1611, 2000). The cDNA's were prepared with an oligo containing a NotI site, and SalI linkers were added to the ends. The inserts were cut with NotI before being cloned into the NotI-SalI sites in the vectors. This is one of two libraries, ngn3 wt and ngn3 -/-. The ngn3 -/- library is in pSPORT2, T7 promoter is 3'.
R. Site1:Not I
R. Site2:Sal I
Lab Host:E. coli-DH12S (GIBCO)
Vector:pSPORT2 (GIBCO)
Vector Type:plasmid
Tissue Supplier:
Library Producer: